In this study semen from Nili Ravi buffalo bulls (n=16) was divided into two groups (each with n=08) on the basis of age factor. Further sub division was done in such a way that every day six bulls were used in collection and evaluation and subjected to the different inclusion levels of BHT @ 0.5mM, I.0mM, 1.5Mm, 2.0mM, 2.5mM, 3....